mab 2g12 Search Results


2g12 mab  (Greiner Bio)
90
Greiner Bio 2g12 mab
(A) Monolayers of HEK 293T cells were infected with ChAdOx1.BG505s at the indicated MOIs for 48 h. The proteins in the tissue culture supernatants were separated by non-denaturing polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. BG505 Env proteins were detected using the glycan-specific <t>2G12</t> mAb as the primary antibody. As a positive control, supernatant from a HEK 293T stable cell line that expresses fully cleaved BG505s trimers was analysed in the first lane. The relative molecular masses of 600 and 450 kDa are indicated. (B) To assess the percentage of correctly folded trimers in the ChAdOx1.BG505s- and MVA.BG505s-infected cell culture supernatants, the ELISA plates were first coated with either 2G12 or PGT151 Env-specific bnAbs and the captured native-like, furin-cleaved trimers were detected using PGT145 as the secondary mAb. To generate the standard curve used for trimer quantification (top), gp140 monomers and trimers purified from stably transfected cells were mixed at different ratios, but at a constant total concentration. The latter values were determined using a different, non-trimer specific capture ELISA (see section). The percentages of native-like BG505s trimer present in the virus-infected cell culture supernatants are shown for the indicated antibody combinations (bottom).
2g12 Mab, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2g12 mab/product/Greiner Bio
Average 90 stars, based on 1 article reviews
2g12 mab - by Bioz Stars, 2026-02
90/100 stars
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mab 2g12  (Corning Life Sciences)
90
Corning Life Sciences mab 2g12
(A) Monolayers of HEK 293T cells were infected with ChAdOx1.BG505s at the indicated MOIs for 48 h. The proteins in the tissue culture supernatants were separated by non-denaturing polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. BG505 Env proteins were detected using the glycan-specific <t>2G12</t> mAb as the primary antibody. As a positive control, supernatant from a HEK 293T stable cell line that expresses fully cleaved BG505s trimers was analysed in the first lane. The relative molecular masses of 600 and 450 kDa are indicated. (B) To assess the percentage of correctly folded trimers in the ChAdOx1.BG505s- and MVA.BG505s-infected cell culture supernatants, the ELISA plates were first coated with either 2G12 or PGT151 Env-specific bnAbs and the captured native-like, furin-cleaved trimers were detected using PGT145 as the secondary mAb. To generate the standard curve used for trimer quantification (top), gp140 monomers and trimers purified from stably transfected cells were mixed at different ratios, but at a constant total concentration. The latter values were determined using a different, non-trimer specific capture ELISA (see section). The percentages of native-like BG505s trimer present in the virus-infected cell culture supernatants are shown for the indicated antibody combinations (bottom).
Mab 2g12, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab 2g12/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
mab 2g12 - by Bioz Stars, 2026-02
90/100 stars
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2g12 mab  (Inserm Transfert)
90
Inserm Transfert 2g12 mab
(A) Monolayers of HEK 293T cells were infected with ChAdOx1.BG505s at the indicated MOIs for 48 h. The proteins in the tissue culture supernatants were separated by non-denaturing polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. BG505 Env proteins were detected using the glycan-specific <t>2G12</t> mAb as the primary antibody. As a positive control, supernatant from a HEK 293T stable cell line that expresses fully cleaved BG505s trimers was analysed in the first lane. The relative molecular masses of 600 and 450 kDa are indicated. (B) To assess the percentage of correctly folded trimers in the ChAdOx1.BG505s- and MVA.BG505s-infected cell culture supernatants, the ELISA plates were first coated with either 2G12 or PGT151 Env-specific bnAbs and the captured native-like, furin-cleaved trimers were detected using PGT145 as the secondary mAb. To generate the standard curve used for trimer quantification (top), gp140 monomers and trimers purified from stably transfected cells were mixed at different ratios, but at a constant total concentration. The latter values were determined using a different, non-trimer specific capture ELISA (see section). The percentages of native-like BG505s trimer present in the virus-infected cell culture supernatants are shown for the indicated antibody combinations (bottom).
2g12 Mab, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2g12 mab/product/Inserm Transfert
Average 90 stars, based on 1 article reviews
2g12 mab - by Bioz Stars, 2026-02
90/100 stars
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anti-hiv mab 2g12  (Technologiefonds)
90
Technologiefonds anti-hiv mab 2g12
(A) Monolayers of HEK 293T cells were infected with ChAdOx1.BG505s at the indicated MOIs for 48 h. The proteins in the tissue culture supernatants were separated by non-denaturing polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. BG505 Env proteins were detected using the glycan-specific <t>2G12</t> mAb as the primary antibody. As a positive control, supernatant from a HEK 293T stable cell line that expresses fully cleaved BG505s trimers was analysed in the first lane. The relative molecular masses of 600 and 450 kDa are indicated. (B) To assess the percentage of correctly folded trimers in the ChAdOx1.BG505s- and MVA.BG505s-infected cell culture supernatants, the ELISA plates were first coated with either 2G12 or PGT151 Env-specific bnAbs and the captured native-like, furin-cleaved trimers were detected using PGT145 as the secondary mAb. To generate the standard curve used for trimer quantification (top), gp140 monomers and trimers purified from stably transfected cells were mixed at different ratios, but at a constant total concentration. The latter values were determined using a different, non-trimer specific capture ELISA (see section). The percentages of native-like BG505s trimer present in the virus-infected cell culture supernatants are shown for the indicated antibody combinations (bottom).
Anti Hiv Mab 2g12, supplied by Technologiefonds, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hiv mab 2g12/product/Technologiefonds
Average 90 stars, based on 1 article reviews
anti-hiv mab 2g12 - by Bioz Stars, 2026-02
90/100 stars
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mab 2g12 antibody  (Aviragen Inc)
90
Aviragen Inc mab 2g12 antibody
(A) Monolayers of HEK 293T cells were infected with ChAdOx1.BG505s at the indicated MOIs for 48 h. The proteins in the tissue culture supernatants were separated by non-denaturing polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. BG505 Env proteins were detected using the glycan-specific <t>2G12</t> mAb as the primary antibody. As a positive control, supernatant from a HEK 293T stable cell line that expresses fully cleaved BG505s trimers was analysed in the first lane. The relative molecular masses of 600 and 450 kDa are indicated. (B) To assess the percentage of correctly folded trimers in the ChAdOx1.BG505s- and MVA.BG505s-infected cell culture supernatants, the ELISA plates were first coated with either 2G12 or PGT151 Env-specific bnAbs and the captured native-like, furin-cleaved trimers were detected using PGT145 as the secondary mAb. To generate the standard curve used for trimer quantification (top), gp140 monomers and trimers purified from stably transfected cells were mixed at different ratios, but at a constant total concentration. The latter values were determined using a different, non-trimer specific capture ELISA (see section). The percentages of native-like BG505s trimer present in the virus-infected cell culture supernatants are shown for the indicated antibody combinations (bottom).
Mab 2g12 Antibody, supplied by Aviragen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab 2g12 antibody/product/Aviragen Inc
Average 90 stars, based on 1 article reviews
mab 2g12 antibody - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


(A) Monolayers of HEK 293T cells were infected with ChAdOx1.BG505s at the indicated MOIs for 48 h. The proteins in the tissue culture supernatants were separated by non-denaturing polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. BG505 Env proteins were detected using the glycan-specific 2G12 mAb as the primary antibody. As a positive control, supernatant from a HEK 293T stable cell line that expresses fully cleaved BG505s trimers was analysed in the first lane. The relative molecular masses of 600 and 450 kDa are indicated. (B) To assess the percentage of correctly folded trimers in the ChAdOx1.BG505s- and MVA.BG505s-infected cell culture supernatants, the ELISA plates were first coated with either 2G12 or PGT151 Env-specific bnAbs and the captured native-like, furin-cleaved trimers were detected using PGT145 as the secondary mAb. To generate the standard curve used for trimer quantification (top), gp140 monomers and trimers purified from stably transfected cells were mixed at different ratios, but at a constant total concentration. The latter values were determined using a different, non-trimer specific capture ELISA (see section). The percentages of native-like BG505s trimer present in the virus-infected cell culture supernatants are shown for the indicated antibody combinations (bottom).

Journal: PLoS ONE

Article Title: HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers

doi: 10.1371/journal.pone.0181886

Figure Lengend Snippet: (A) Monolayers of HEK 293T cells were infected with ChAdOx1.BG505s at the indicated MOIs for 48 h. The proteins in the tissue culture supernatants were separated by non-denaturing polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. BG505 Env proteins were detected using the glycan-specific 2G12 mAb as the primary antibody. As a positive control, supernatant from a HEK 293T stable cell line that expresses fully cleaved BG505s trimers was analysed in the first lane. The relative molecular masses of 600 and 450 kDa are indicated. (B) To assess the percentage of correctly folded trimers in the ChAdOx1.BG505s- and MVA.BG505s-infected cell culture supernatants, the ELISA plates were first coated with either 2G12 or PGT151 Env-specific bnAbs and the captured native-like, furin-cleaved trimers were detected using PGT145 as the secondary mAb. To generate the standard curve used for trimer quantification (top), gp140 monomers and trimers purified from stably transfected cells were mixed at different ratios, but at a constant total concentration. The latter values were determined using a different, non-trimer specific capture ELISA (see section). The percentages of native-like BG505s trimer present in the virus-infected cell culture supernatants are shown for the indicated antibody combinations (bottom).

Article Snippet: The 2G12 mAb (2.0 μg/ml) was coated onto ELISA plates (Greiner Bio-One) at 4°C during an overnight incubation.

Techniques: Infection, Polyacrylamide Gel Electrophoresis, Positive Control, Stable Transfection, Cell Culture, Enzyme-linked Immunosorbent Assay, Purification, Transfection, Concentration Assay